Phenotypic characterization of gavi. Whole mount in situ hybridization for transferrin-a (A-D), TfR2 (E-H), hepcidin (I-R), and LFABP (S-V) for WT siblings (A,B,E,F,I,J,M,O,P,S,T) versus gavIT029 (C,D,G,H,K,L,N,Q,R,U,V) in dorsal and lateral views at 52 hpf (A-N,S-V) and 72 hpf (O-R). White arrow indicates the liver. Black arrow indicates the foregut. Red arrow indicates the proctodeum (terminal gut). The scale bar represents 100 μm.