Changes in gene expression in gavi and other hypochromic mutants. At 52 hpf, zebrafish embryos were either injected with iron dextran or anesthetized, but not injected. RNA was obtained from pools of anemic mutants and their wild-type siblings at 72 hours after fertilization, 18 hours after iron injection. Quantitative multiplex real-time RT-PCR, normalized to β-actin expression, was performed for transferrin-a (A), fpn1 (B), and hepcidin (C-E) in gav^IT029 (A-C), TfR1a-deficient chianti (cia^Tu25f) mutants (D) and DMT1-deficient chardonnay (cdy^Te216) mutants (E). Gene expression is reported as fold increase over calibrator, a WT uninjected control pool. *P < .05 compared with WT uninjected controls.