CD8+ T-cell responses defined by analysis of intracellular cytokine production. (A) AML patient 3. (B) CML patient 17. (C) ALL patient 23. (D) Healthy donor 34. PBMCs (106) were loaded with test peptides, and intracellular cytokine analysis for IFN-γ was performed as described in “Methods.” Plots are gated on CD3+ T cells. Numbers in the upper right quadrant represent the percentage of IFN-γ–producing CD8+ T cells.