Treg increase in CpG/CTX-vaccinated mice and preferential T_eff proliferation during immunotransplantation. (A) Mice received CpG/CTX vaccination as described. (B) On day 15 after vaccination, donor mice splenocytes were assessed for T-cell subsets by flow cytometry. (C) Splenocytes were taken from wild-type donor mice and labeled with 5 μg/mL CFSE and injected along with unlabeled BM cells into recipients that received either no (0 cGy) or lethal (900 cGy) TBI. (D) On day 15 after transplantation, splenocytes were taken from 3 recipient mice and separately measured by flow cytometry for CFSE, surface CD4, and intracellular foxP3. Data shown are gated on live, CFSE⁺, CD4⁺ cells. Data shown are representative of the 3 individual recipients.