TNF-α induces aberrant JNK activation in Fancc^−/− MEFs. (A) JNK activity assays. WT and Fancc^−/− MEFs were either untreated or treated with 50 ng/mL TNF-α for 30 minutes before conducting JNK in vitro kinase assays. Autoradiography of a representative JNK kinase assay is shown. (B) Mean densitometry data for JNK kinase experiments are shown; n = 4. *P < .05.