JNK inhibition protects Fancc^−/− MEFs from TNF-α-induced apoptosis. (A) JNK inhibitor and apoptosis assays. WT and Fancc^−/− MEFs were either grown in normal conditions or pretreated with JNK III before 50 ng/mL TNF-α treatment. Apoptosis was analyzed by TUNEL; n = 3, *P < .003. (B) JNK inhibitor and activity assays. WT and Fancc^−/− MEFs were either untreated or treated with 50 ng/mL TNF-α for 30 minutes in the presence or absence of JNK III before conducting JNK in vitro kinase assays. Autoradiography of a representative JNK kinase assay of 3 independent experiments is shown. (C) JNK siRNA Western blot. WT and Fancc^−/− MEFs were transfected with either a pool of JNK1 or control siRNA oligonucleotides. Forty-eight hours after transfection, cells were used for Western blotting and for culture with 50 ng/mL TNF-α overnight. Representative JNK and β-actin Western blots are shown. (D) JNK siRNA apoptosis assays. Data shown are the mean of 3 independent transfection experiments with similar results. *P < .01.