Flow cytometric analysis of Crry expression and C3 deposition on WT and mutant mouse thymocytes and peripheral T and B cells. (A) Analysis of thymocytes in CD4-Cre⁻-Crry^flox/flox and CD4-Cre⁺-Crry^flox/flox mice. (B) Analysis of thymocytes in Lck-Cre⁻-Crry^flox/flox and Lck-Cre⁺-Crry^flox/flox mice. (C,E) Analysis of CD4⁺ and CD8⁺ T lymphocytes (C) and B220⁺ B lymphocytes (E) in CD4-Cre⁻-Crry^flox/flox and CD4-Cre⁺-Crry^flox/flox mice. (D,F) Analysis of CD4⁺ and CD8⁺ T lymphocytes (D) and B220⁺ B lymphocytes (F) in Lck-Cre⁻-Crry^flox/flox and Lck-Cre⁺-Crry^flox/flox mice. In panels E and F, open histogram represents Cre(-) and filled histogram represents Cre(+) genotype (the 2 histograms completely overlap), respectively. The absorbed antibody, α-Crry3/4, was used for all Crry staining.