Analysis of the HA-1 TSS and promoter. (A) Depicted is the restriction map of the intergenic region between the ABCA7 gene and the HA-1 gene. The transcription start site (TSS) of the HA-1 gene was determined by 5′ RACE analysis and located −239 bp 5′ of the HA-1 translation start site (ATG). (B) The presence of promoter activity in proximity to the TSS was investigated with deletion constructs of the intergenic region. Depicted are the constructs fused to luciferase reporter (pGL3-Basic). Luciferase activity was determined on transient transfection of the deletion constructs into Raji; x-axis indicates that results were normalized to pGL3-Basic (relative light unit) and are shown as the mean (±SD) of 3 independent experiments.