5-AZA-CdR induces both HA-1 alleles in HA-1^neg tumor cells. mRNA expression of the HA-1 H and R alleles was determined by allele-specific qPCR. The x-axis indicates 518A2 melanoma cells before and after treatment with 100 μM 5-AZA-CdR for 8 days, MDA-MB 231 tumor cells and control EBV LCLs (HA-1H/R, HA-1R/R, and HA-1HH); y-axis, mRNA expression levels of the HA-1 H and R alleles in relation to the expression levels in MDA-MB 231. EBV LCLs indicate Epstein-Barr virus–transformed B cells. Error bars reflect SEM. Values for gene expression that do not show error bars reflect data in which the SEM was too small to be depicted graphically.