Assessment of proliferation after silencing of selected targets. A total of 2 unique siRNA sequences were designed and synthesized against each of 20 selected transcripts derived from the DNA microarray study. Granta 519 cells were nucleofected with the siRNAs and appropriate control siRNAs, and the proliferative effect of the sequence-specific knockdown was assessed. Electroporation control (ie, electroporation without any siRNA) was set to 100%. Bars represent plus or minus SD of triplicate samples.