Mll regulates mir-196b expression in hematopoietic progenitors. (A) RT-PCR for canonical Hoxa9 in CD41⁺ ES cells. Day 10 EBs were disrupted and CD41⁺ cells isolated using a magnetic column. Total RNA was isolated from the CD41⁺ cells and used for cDNA synthesis. Hoxa9 primers used span the junction between exon CD and exon II of the gene. Gapdh is used as a loading control. Space between panels indicates repositioned gel lanes. (B) Quantitative RT-PCR of mir-196b expression in WT and Mll^−/−CD41⁺ and CD41⁻ murine embryonic stem cells from day 10 embryoid bodies. WT levels were set to 1 for comparison. Experiment was performed in triplicate and the results represent average expression plus or minus SD. (C) Quantitative RT-PCR of mir-196b expression in sorted mouse bone marrow cells. Mouse bone marrow cells were sorted in various populations based on the expression of cell surface markers. Expression of LT-HSCs is set to1 for comparison. The results represent average fold change plus or minus SD.