MLL fusion proteins induce expression of mir-196b in primary bone marrow progenitors, which is required for their MLL fusion-dependent increased proliferative capacity in vitro. Wild-type Lin⁻ bone marrow cells were transduced with MLL-AF9 retroviral vectors and treated with either control antagomiR-196 or antagomiR-196, then plated in duplicate. Colonies were enumerated after 8 days and the average number was plotted. Cells were then replated after additional antagomir treatment. The cycle was repeated twice. Data shown are representative of 2 independent experiments with similar results.