American Society of Hematology

Figure 3

$Figure 3. ETS2, ERG, and FLI-1 promote megakaryopoiesis in Gata1s knockin progenitors. (A) Gata1s knockin fetal liver cells transduced with MIGR1, MIGR1-ETS2, MIGR1-ERG, or MIGR1-FLI-1 were differentiated for 72 hours and the GFP+ fraction was assessed for CD41 expression by flow cytometry. (B) The degree of polyploidization was determined by DAPI staining of GFP, CD41 double-positive differentiated fetal liver cells. (C) CD42 expression within the GFP-positive population was assessed by flow cytometry. (D) The degree of polyploidization was determined by DAPI staining of GFP, CD42 double-positive differentiated fetal liver cells. Fold changes for CD41 (E), CD42 (F) expression, and percentage of cells with DNA content of 8N or more (G) in the transduced cells relative to MIGR1 control-infected cells are shown below the representative flow plots. Graphs display average fold changes plus or minus SE. * P less than or equal to .03. For all experiments, n = 3.$

ETS2, ERG, and FLI-1 promote megakaryopoiesis in Gata1s knockin progenitors. (A) Gata1s knockin fetal liver cells transduced with MIGR1, MIGR1-ETS2, MIGR1-ERG, or MIGR1-FLI-1 were differentiated for 72 hours and the GFP⁺ fraction was assessed for CD41 expression by flow cytometry. (B) The degree of polyploidization was determined by DAPI staining of GFP, CD41 double-positive differentiated fetal liver cells. (C) CD42 expression within the GFP-positive population was assessed by flow cytometry. (D) The degree of polyploidization was determined by DAPI staining of GFP, CD42 double-positive differentiated fetal liver cells. Fold changes for CD41 (E), CD42 (F) expression, and percentage of cells with DNA content of 8N or more (G) in the transduced cells relative to MIGR1 control-infected cells are shown below the representative flow plots. Graphs display average fold changes plus or minus SE. * P less than or equal to .03. For all experiments, n = 3.

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