American Society of Hematology

Figure 6

$Figure 6. Differentially active forms of trimeric and oligomeric BAFF in mouse plasma. Plasma from BAFF transgenic (A), wild-type (B), and TACI−/− (C) mice were fractionated by gel permeation chromatography. Mouse BAFF was quantified in the fractions by ELISA (■) and expressed with different scales for each genotype. The oligomerization-dependent activity of BAFF in 25 μL, 5 μL, and 2.5 μL of wild-type, BAFF transgenic, and TACI−/− fractions, respectively, was detected with reporter Jurkat BCMA:Fas cells that die in response to oligomerized BAFF (○). For wild-type and TACI−/− fractions, the assay was performed in the presence of cycloheximide (CHX) that enhances the sensitivity of the assay. Where indicated, BCMA-Fc was added to demonstrate the killing specificity (●).$

Differentially active forms of trimeric and oligomeric BAFF in mouse plasma. Plasma from BAFF transgenic (A), wild-type (B), and TACI^−/− (C) mice were fractionated by gel permeation chromatography. Mouse BAFF was quantified in the fractions by ELISA (■) and expressed with different scales for each genotype. The oligomerization-dependent activity of BAFF in 25 μL, 5 μL, and 2.5 μL of wild-type, BAFF transgenic, and TACI^−/− fractions, respectively, was detected with reporter Jurkat BCMA:Fas cells that die in response to oligomerized BAFF (○). For wild-type and TACI^−/− fractions, the assay was performed in the presence of cycloheximide (CHX) that enhances the sensitivity of the assay. Where indicated, BCMA-Fc was added to demonstrate the killing specificity (●).

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