IUT decreases growth plate height. (A) Tibial growth plate analysis in 4-week-old (OIM + IUT, n = 7; OIM, n = 12), 8-week-old (OIM + IUT, n = 9; OIM, n = 11), and 12-week-old (OIM + IUT, n = 3; OIM, n = 5) mice. The heights of the total GP, RZ, PZ and HZ are shown (± SE). **P < .01; or ***P < .001. (B) Tibial sagittal sections showing growth plates in 4-, 8- and 12-week-old OIM + IUT and OIM mice. (C) Femur sagittal sections under the growth plate showing trabeculae and calcified cartilage (magnified inserts) in OIM versus OIM + IUT mice at 4, 8, and 12 weeks of age. Magnification was 200×.