American Society of Hematology

Figure 1

$Figure 1. CREB expression in mouse and human hematopoietic progenitors. (A) CREB expression levels in lin− and lin+ cells as measured by q-PCR. CREB expression was higher (2.6-fold) in the lin− population than the lin+ population (P = .01). (B) Gating strategy for cell sorting of lin− cells to isolate HSC (Ba), CMP (Bb), GMP (Bc), MEP (Bd), CLP (Be), LT-HSC (Bf), ST-HSC (Bg), and MPP (Bh). (C) cDNA from HSC CMPs, CLPs, GMPs, and MEPs were confirmed by expression of SCL, c-Mpl, Pu-1, Aiolos, and CEBPα. (D) CREB expression levels in HSC, CMP, GMP, MEP, and differentiated cells. Compared with mature cells, CREB was 2.5- to 4-fold higher in HSC, CMP, GMP, and MEP. (E) CREB expression in LT-HSC, ST-HSC, and MPP. CREB was slightly higher (4- vs 3-fold) in the MPP fraction compared with the LT-HSC and ST-HSC populations, but this was not found to be statistically significant (P = .06). CREB expression in human lin− and lin+ cells isolated from cord blood (F) or peripheral blood (G) stem cells. CREB expression was higher for both cell types in the lin− population than the lin+ population (P = .01). (H and I) CREB expression in human bone marrow cells. CREB was also expressed at higher levels in CD34+ CD38− cells (b) than CD34+ CD38+ cells (a). All experiments were performed in triplicate. Error bars represent SE.$

CREB expression in mouse and human hematopoietic progenitors. (A) CREB expression levels in lin⁻ and lin⁺ cells as measured by q-PCR. CREB expression was higher (2.6-fold) in the lin⁻ population than the lin⁺ population (P = .01). (B) Gating strategy for cell sorting of lin⁻ cells to isolate HSC (Ba), CMP (Bb), GMP (Bc), MEP (Bd), CLP (Be), LT-HSC (Bf), ST-HSC (Bg), and MPP (Bh). (C) cDNA from HSC CMPs, CLPs, GMPs, and MEPs were confirmed by expression of SCL, c-Mpl, Pu-1, Aiolos, and CEBPα. (D) CREB expression levels in HSC, CMP, GMP, MEP, and differentiated cells. Compared with mature cells, CREB was 2.5- to 4-fold higher in HSC, CMP, GMP, and MEP. (E) CREB expression in LT-HSC, ST-HSC, and MPP. CREB was slightly higher (4- vs 3-fold) in the MPP fraction compared with the LT-HSC and ST-HSC populations, but this was not found to be statistically significant (P = .06). CREB expression in human lin⁻ and lin⁺ cells isolated from cord blood (F) or peripheral blood (G) stem cells. CREB expression was higher for both cell types in the lin⁻ population than the lin⁺ population (P = .01). (H and I) CREB expression in human bone marrow cells. CREB was also expressed at higher levels in CD34⁺ CD38⁻ cells (b) than CD34⁺ CD38⁺ cells (a). All experiments were performed in triplicate. Error bars represent SE.

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