Organization of the kininogen gene in mice. (A) Map of the DNA fragment of murine BAC clone RP23–452G1 containing mKng1 and mKng2. Exons are denoted by numbered boxes, and arrows indicate the direction of gene transcription. (B) Alignment of the mKng1 and mKng2 cDNA regions that were used for RT-PCR amplification. The amplified region spanned the carboxyl-terminal region of domain 3 through the amino-terminal region of domain 6. The 2 gaps in mHK2 responsible for the different sizes of the PCR products from the 2 cDNAs are denoted by dashes. Also shown are the corresponding polypeptide sequences of the mHK1 and mHK2 proteins. The ERDAETEQGPTH sequence used to raise the mHK1-specific antibody is underlined.