RAD001 promotes autophagy and induces apoptosis. Ultrastructural analysis by TEM of ultrathin BM sections in mice treated with vehicle (A), vincristine (B), RAD001 (C), and vincristine plus RAD001 (D). Apoptotic cells (), extravasated erythrocytes (*), filipodia (white arrows), and phagolysosomes (white arrowheads) are indicated. Double-walled polyphagic vacuoles in the cytoplasm of ALL cells from ALL-1345 (E) and ALL-1999 (F,G) recovered from mice treated with RAD001. Bars represent 1 μm. See “Electron microscopy” for details. Larger images are available in Figure S4.