CCND1 is a target of miR-16-1 as predicted by Target Scan. (A) Shown are the seed binding regions located in the distal region of the CCND1 3′ UTR. The 2 binding sites for miR-16-1 are boxed. The truncation (shown by ↑) deletes CCND1 all miRNA binding sites. (Adapted from Target Scan.²¹ ) (B) Table showing the context percentile score of each microRNA family predicted to target CCND1 by Target Scan.²¹  The higher the context score, the higher the probability that the microRNA will be able to inhibit CCND1 translation. (C) qRT-PCR to show the endogenous miR-16-1 expression in MCL cell lines. Error bars represent SD.