Expression of TLR2 and TLR4 by human eosinophils. (A) Surface expression of TLR2 and TLR4 on human eosinophils incubated with or without BCG (10:1). Results correspond to one representative normal donor (ND, no. 13 of Table S1) and eosinophilic donor (ED, no. 5 of Table S1). Gray histogram and black line represent staining with specific and isotype control antibodies, respectively. The data are displayed by using a logarithmic scale and the change in mean fluorescence intensity (ΔMFI: values from isotype control antibodies are subtracted from values obtained with each condition) are indicated in the plot. (B) Cell-surface TLR2 and TLR4 expressions on purified eosinophils from 16 individual eosinophil donors stimulated with or without BCG (10:1). Group average expression for the indicated markers is represented (−).*P < .05.