Involvement of TLR2 in the activation of eosinophils by M bovis BCG. (A) Kinetics of BCG-induced ROS release (ratio 10:1, ■) inhibited by an anti-TLR2 blocking antibody 20 μg/mL (▴), 10 μg/mL (*), or an isotype control antibody 20 μg/mL (□). Spontaneous ROS release by eosinophils is indicated (−). Results correspond to 1 representative of 3 experiments and are expressed in counts per second (cps). (B) BCG-induced ROS production (ratio 10:1, ■) inhibited by an anti-TLR4 blocking antibody 20 μg/mL (▴), 10 μg/mL (*), or an isotype control antibody 20 μg/mL (□). Spontaneous ROS release of eosinophils is indicated (−). Results are expressed as ROS cps values and one representative of 3 experiments was shown. (C) Inhibition of BCG-induced EPO release by anti-TLR2 and anti-TLR4 blocking antibodies (20 μg/mL) or an isotype control antibody. ΔEPO cps values (values from medium stimulation are subtracted from values obtained with each antibody). Results are expressed as mean plus or minus SEM (n = 5-6). (D) LM (1 μg/mL)–induced EPO release inhibited by anti-TLR2 or anti-TLR4 blocking antibodies (20 μg/mL) or an isotype control antibody. Results are expressed as ΔEPO cps values and as mean plus or minus SEM (n = 5). *P < .05.