BCG growth inhibition by eosinophils. (A) BCG growth in the presence of eosinophils for the indicated times. Data are shown as the mean of 3 independent experiments plus or minus SEM, expressed in percentage of CFU control ([CFU with eosinophils/CFU with medium] ×100). (B) Quantitive detection of internalized PKH26-BCG by fluorescence microscopy. Eosinophils were incubated with or without BCG for 40 minutes at 37°C. (C) Intracellular BCG growth inhibition with different BCG/eosinophil ratios (5:1, ■; 10:1, ▵; 20:1, ●). Results are expressed as mean plus or minus SEM (n = 3). Significant differences between conditions studied and controls are indicated as followed: *P < .05, **P < .01.