The Fpn disease mouse model ffe/+ macrophages show increased C psittaci infections. (A,B) Wild-type (C3H) and ffe/+ mouse bone marrow macrophages were incubated with iron for 48 hours, infected with C psittaci for 2 hours, incubated with or without hep25 for 18 to 24 hours, and processed for C psittaci immunofluorescence as in Figure 1. (C) Ferritin levels were determined from cells infected as in panel A and then incubated with hep25, hep20, hep25 and deferriprone, or hep25 and desferasirox. Fpn levels were detected by Western blot using a rabbit anti–mouse Fpn followed by a peroxidase conjugated goat anti–mouse IgG. Error bars represent SD. (D) Bone marrow macrophages from ffe/+ mice treated as in panel C were processed for immunofluorescence. Bar represents 10 μm. Experiments were performed a minimum of 3 times and the error bars represent the standard deviation of the mean.