Iron chelators deferriprone and desferasirox remove iron stored in macrophages limiting intracellular L pneumophila growth. Bone marrow macrophages isolated from A/J mice were iron loaded as in Figure 1, infected with L pneumophila for 2 hours, and incubated with hepcidin with or without iron chelators deferriprone or desferasirox for 18 hours. Cells were either (A) lysed and lysates plated on CYET for 3 days and CFUs determined or (B) processed for immunofluorescence and bacterial foci/cell determined as described in Figure 2. Experiments were repeated a minimum of 3 times. Error bars represent the standard deviation of the mean.