Relative expression of Wnt/Fzd components during malignant transformation. The relative mRNA levels of Fzd6, Wnt16, Wnt10a, LRP5, and LRP6 (A), of transcription cofactors β-catenin, LEF-1, and TCF-1 (B), and of Wnt/β-catenin pathway target genes Myc, Jun, Cyclin D3, and VEGF-α (C) were determined using real-time PCR. Expression levels in flow-sorted oligoclonal CD19⁺CD5⁺Igλ⁻ tumor B cells (●; same as population B in Figure 1) and monoclonal CD19⁺CD5⁺Igλ⁺ B tumor B cells (■; same as population C in Figure 1) are depicted, relative to levels in normal CD19⁺CD5⁻ B cells (same as population A in Figure 1) from the same animals. The relative expression levels were determined by normalizing the ΔCt values of the tumor population against the ΔCt values for normal CD19⁺CD5⁻ B cells for the specific genes from the specific mouse. The solid lines connect the oligoclonal and monoclonal tumor cell populations from the same mouse. All data are shown, but in some cases there was inadequate mRNA obtained to do all analyses. By 2-sample, equal variance t test, Fzd6 expression is significantly increased between CD5⁻ B cells and CD5⁺ oligoclonal B cells (P = .027) and CD5⁺ monoclonal B cells (P = .006). Other consistently increased genes, such as Wnt10a and Wnt16, did not reach statistical significance.