Impact of Fzd6 KO on the expression of Wnt, Fzd, and other Wnt pathway components on Eμ-TCL1–induced tumor B cells. Total RNA was isolated from flow cytometry–sorted CD19⁺CD5⁺ tumor B cells of age and tumor burden matched Eμ-TCL1^+/−Fzd6^−/−, Eμ-TCL1^+/−Fzd6^+/−, and Eμ-TCL1^+/−Fzd6^+/+ tumor-bearing mice (n = 2). Gene expression of Wnt, Fzd families, and other components related with the Wnt pathway was determined by real-time PCR array of mouse Wnt signaling as described in “Real-time PCR array.” Data are shown as relative expression levels determined by normalizing the ΔCt values of the Eμ-TCL1^+/−Fzd6^−/− or Eμ-TCL1^+/−Fzd6^+/− against the ΔCt values for Eμ-TCL1^+/−Fzd6^+/+ mice for the specific genes. The genes, which on average show no less than 4-fold changes in both Eμ-TCL1^+/−Fzd6^−/− and Eμ-TCL1^+/−Fzd6^+/− mice, are indicated in the plot.