Runx2 overexpression alters expression of CBF targets and myeloid differentiation. (A) RELs of CBF targets Csf1r, Mpo, Cebpd, and Cdkn1a, and myeloid genes Cebpa and Gfi1 in GFP-sorted wt and CM progenitors infected with MIG or Runx2 (Rx2) retrovirus, using quantitative RT-PCR assay. Data are mean plus or minus SD from at least 2 independent experiments, each in triplicate. (B) RELs of CBF targets Cebpd and Cdkn1a, in GFP-sorted wt and CM progenitors infected with MIG, Runx2 (Rx2), or Runx2 Y418A (Y418) mutant retrovirus, using quantitative RT-PCR assay. (C) Chromatin immunoprecipitation assay of cells expressing Runx2 using primers for promoter sequences (horizontal double line) of Csf1r, Mpo, Cebpd, and Cdkn1a (left) with CBF consensus sites (vertical line), and CBF-unrelated control genes Cdc6 and Phox, using anti-Runx2 (■) or anti-IgG antibody. Relative specificity is shown as percentage of input.