Comparison of innate immune response initiated by HF-HSV and H+-HSV in CLL B cells and THP1 cells. (A-C) CLL B cells (A,B) and THP1 cells (C) were transduced with HF-HSV or H+-HSV amplicons at the indicated MOI; 8 hours later (A,C) innate response was assayed by qRT-PCR for TNF-α, IL6, IP10, and IFN-β mRNA. AU indicates arbitrary units. Data are representative of 3 experiments. *P < .05. In panel B, CLL B cells were transduced with HF-HSV or H+-HSV amplicons at MOI of 0.5, and TNF-α and IP10 response were assayed by qRT-PCR at multiple time points as indicated on the x-axis. (D) Comparison of innate response to HF-HSV, H+-HSV, and the HSV helper virus component of H+-HSV in THP1 cells at MOI of 0.5, 8 hours after transduction, shows that HF-HSV generates more potent innate response than either H+-HSV or HSV helper virus alone. Data are representative of 2 experiments. Error bars represent SEM.