Changes in the cytokine milieu of the peripheral blood from patients before and after vaccination. To screen type 1 and type 2 T-cell activation, we measured the levels of interleukin-2 and IL-10 (IL-2 and IL-10, respectively) in the sera of all 10 patients before and 3 weeks after the last of 4 RHAMM-R3 peptide vaccinations. While IL-10 levels remained rather low, an up-to-5-fold increase in IL-2 levels as well as stable or decreasing levels for the cytokine were observed. Patient numbers indicated below the columns refer to the patient numbers in Table 1. Error bars represent SD.