Identification of the Ank-1¹⁶⁷⁴ allele. (A) Haplotypes of F₂ mapping offspring. Markers used and their position on mouse chromosome 8 according to the July 2007 University of California Santa Cruz genome sequence (based on the National Center for Biotechnology Information [NCBI] and Mouse Genome Sequencing Consortium Build 37) are shown. [□] indicates homozygous BALB/c genotype; [], homozygous C57BL/6, and [], heterozygous. The candidate interval for RBC2 was refined to 11 Mb, between D8Mit124 and D8Mit3. Ank-1 lies at 24 085 316-24 260 968 Mb. (B) DNA sequence electropherograms showing the G to C transversion at the splice acceptor site of exon 41 in Ank-1^1674/+ and Ank-1^1674/1674 mice. (C) Schematic view of the predicted wild-type and Ank-1¹⁶⁷⁴ alleles. (D) Schematic of the wild-type and the predicted Ank-1¹⁶⁷⁴ proteins.