Cytokine levels during the course of the air-pouch model of acute inflammation in mice. (A) Scheme of the air-pouch model of local inflammation as outlined in “Air-pouch model.” (B) Levels of endogenous IL6 in inflamed and noninflamed air pouches at different time points were measured by enzyme-linked immunosorbent assay (ELISA). (C) Levels of endogenous sIL6R in inflamed and noninflamed air pouches at different time points were measured by ELISA. In noninflamed air pouches, IL6 and sIL6R were hardly detectable. Four to 7 mice per time point. Data are represented as mean values plus or minus standard deviation (**P < .01; ***P < .05).