Reduced cell infiltration in opt_sgp130Fc transgenic mice during acute inflammation. (A) Levels of transgenic sgp130Fc-protein in inflamed air pouches of opt_sgp130Fc transgenic mice. 72 hours after carrageenan stimulation, the levels of sgp130Fc-protein were assessed by ELISA. Data are represented as mean values plus or minus SD of 8 animals. (B) Quantitative flow cytometry analysis showing inflammatory cells (x10⁶ per pouch) of homozygous opt_sgp130Fc transgenic and WT animals (wt) infiltrating the air pouch 4 hours, 12 hours, and 72 hours after injection of 1% carrageenan. (C) Neutrophils (x10⁶/pouch) infiltrating the air pouch 4 hours, 12 hours, and 72 hours after injection of 1% carrageenan as measured by flow cytometry. (D) Monocytes (x10⁶/pouch) infiltrating the air pouch 4 hours, 12 hours, and 72 hours after injection of 1% carrageenan as measured by flow cytometry. Four to 7 mice per group. Data are represented as mean values plus or minus SD (**P < .01; *P < .05). (E) Quantitative flow cytometry analysis showing inflammatory cells (x10⁶/pouch) infiltrating the air pouch 72 hours after injection of 1% carrageenan. Comparison of WT animals treated with phosphate-buffered saline (PBS), 100 μg sgp130Fc, or 100 μg anti-IL6R mAb 6 hours before carrageenan injection. (F) Neutrophils (x10⁶/pouch) infiltrating the air pouch 72 hours after injection of 1% carrageenan as measured by flow cytometry. Comparison of WT animals administered with PBS, sgp130Fc, or anti-IL6R mAb 6 hours before carrageenan injection. (G) Monocytes (x10⁶/pouch) infiltrating the air pouch 72 hours after injection of 1% carrageenan as measured by flow cytometry. Comparison of WT animals administered with PBS, sgp130Fc, or anti-IL6R mAb 6 hours before carrageenan injection. (H) Levels of recombinant sgp130Fc-protein in serum (μg/mL) and in inflamed air pouches (μg/pouch) of WT mice 78 hours after administration of sgp130Fc. Sgp130Fc-protein levels were assessed by ELISA. Data are represented as mean values plus or minus SD of 5 animals (**P < .01). (I) Neutrophils and monocytes (x10⁶/pouch) infiltrating the air pouch 12 hours after injection of 1% carrageenan as measured by flow cytometry of IL6^−/− and WT animals. Data are represented as mean values plus or minus SD of 3 animals (***P < .015).