Human eosinophils express Aph-1. Freshly isolated human blood eosinophils were analyzed for intracellular Aph-1 expression by flow cytometry of saponin-permeabilized cells (A) and immuno-EM (B,C). Aph-1 expression was observed within the Golgi (Bi), within granules (Bii), and at the cell periphery (Ci). Bars, 870 nm (B,C); 440 nm (Bi,ii and Ci). Nu indicates nucleus. Scale bar equals 1.2 cm.