Enrichment of Ly-6C⁺ cells in the circulating monocyte pool enhances EAE. (A) The absolute number of total monocytes and Ly6C⁺ monocytes per milliliter of blood in adoptive transfer recipients at serial time points after treatment with clodronate liposomes. (B) The absolute number of circulating Ly-6C⁺ monocytes per milliliter of blood 5 days after treatment with either clodronate or PBS liposomes. Mean (± SD) of 5 independent experiments; **P < .01 comparing frequency of Ly-6C⁺ monocytes in PBS versus clodronate treated mice. (C) Clinical course of mice treated with a single dose of clodronate or PBS liposomes 18 hours after the adoptive transfer of encephalitogenic cells. Mice in a third group received clodronate liposomes on days 8, 10, and 12 after T-cell transfer. Data shown represent the results from 3 separate experiments. Mean (± SD) of 3 independent experiments; P < .05 by comparison to PBS treated group.