NK1R and CD40 signaling act synergistically to promote BMDC survival. (A) Bar-diagram representing the percentage of CD11c⁺ BMDCs expressing Bcl-2 after culture in medium alone or with SarSP for different time points. Agonistic CD40 mAb was added at 24 hours to cultures, and the percentage of CD11c⁺ BMDCs expressing Bcl-2 was analyzed at each time point. *P < .05, **P < .01, and ***P < .001. Means (± SD) of 3 independent experiments are shown. (B) Contour-plots illustrating the presence of SarSP-TN-DCs or TN-DCs (CD11c⁺CFSE⁺) in DLNs 5d after adoptive transference of DCs in B6 mice treated with or without the CD154 (CD40L) neutralizing MR1 mAb. Data are representative of 3 independent experiments.