Figure 4
![Figure 4. APO866-mediated cell death involves autophagy. ML-2, Namalwa, Jurkat, or primary AML cells pretreated with either inhibitors of autophagy (wortmannin [WMN], LY294002, 3-methyladenine [3-MA], or bafilomycin A1 [BAFA]) or inhibitor of translation cycloheximide (CHX) were exposed to 10 nM APO866 for either 72 hours (A-D) or 96 hours (E-H). APO866-induced cell death was assessed as described in Figure 1. Percent cell death induced by drug = [(S-C) / (100-C)] × 100; where S = treated sample cell death and C = untreated sample cell death. Data are derived from at least 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/14/10.1182_blood-2008-08-173369/4/zh80170934250004.jpeg?Expires=1735591686&Signature=BC6o1HLiGIFbKXj3ufWJtX5hFTqqwvPVy5RszQ4W7~6qyXx9SgTQM2D831MYiXACJAIEWitOpdCAUZkKiD~uS9R7xNYTlKlH7qWFnqiVua7UYqu7uuLQaoIPcjQWJNVff2-Rd7voJHd~fzuPOXVO-qRS-eq62bFYAAjeIkzUtFGUVCBSee0xOOsfzpJ2vVQ3yVOwcd5b57ctrSLyJeKGN~dqintz4sSAeOYbNvlH2al2TojNNXFAL~voRjAhgRpT5SpONQU8FhWvkH7RS5PGY2O9EPwcuMygGayxZJomIv4-k~2HKvEZyaVKTPf2EnypmOWWnHU13D-ZS2E9dw1Tcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
APO866-mediated cell death involves autophagy. ML-2, Namalwa, Jurkat, or primary AML cells pretreated with either inhibitors of autophagy (wortmannin [WMN], LY294002, 3-methyladenine [3-MA], or bafilomycin A1 [BAFA]) or inhibitor of translation cycloheximide (CHX) were exposed to 10 nM APO866 for either 72 hours (A-D) or 96 hours (E-H). APO866-induced cell death was assessed as described in Figure 1. Percent cell death induced by drug = [(S-C) / (100-C)] × 100; where S = treated sample cell death and C = untreated sample cell death. Data are derived from at least 3 independent experiments.
