Flow cytometric analysis of BM from primary transplant recipients. (A) Whole BM of diseased mice was gated based on forward and side scatter characteristics and subsequently analyzed for expression of hCD4 (TEL-PDGFβR) and EGFP (AML1-ETO). EGFP⁺hCD4⁺ cells were then analyzed for the expression of myeloid and progenitor markers. Plots are from a single animal that is representative of the experimental group. (B) Differences in the percentage of EGFP⁺hCD4⁺ BM cells that were positive for both c-kit and Sca-1. Error bars indicate 95% confidence intervals. AE + TP, n = 21 mice; R174Q + TP, n = 22; Y113A/T161A, n = 24; TP, n = 8; AE, n = 8. Differences relative to AML1-ETO plus TEL-PDGFβR (#) for each group were determined using Dunnett test and ANOVA. (C) Differences in the percentage of EGFP⁺hCD4⁺ BM cells that were positive for both Gr-1 and Mac-1. Error bars indicate 95% confidence intervals. Differences relative to AML1-ETO plus TEL-PDGFβR (#) for each group were determined using Dunnett test and ANOVA. The percentage of Gr-1⁺Mac-1⁺ cells in mice transplanted with AML1-ETO (Y113A/T161A) plus TEL-PDGFβR transduced cells was significantly lower (P < .001) than in mice transplanted with BM expressing AML1-ETO (R174Q) plus TEL-PDGFβR or TEL-PDGFβR alone.