Figure 5
Figure 5. Cbfβ-SMMHC hinders Rag1 and Rag2 expression, and V(D)J recombinase activity. (A) Relative expression levels of Rag1 and Rag2 in sorted CLPs by qRT-PCR. Shown are mean plus or minus SD (n = 3; *** P < .001) from at least 2 independent sorting experiments. (B) Illustration of the transgene H2-SVEX used in this study. The VEX reporter (white rectangle) in the antisense orientation flanked by V(D)J recombination signal sequences (triangles) that direct inversional recombination is driven by the murine H2K promoter (black rectangle). (C) Analysis of recombination reporter VEX expression in BM CLP populations from Cbfb+/56M/Mx1Cre/H2-SVEX mice (restored) or control mice. (D) Analysis of recombination reporter VEX expression in BM pre–pro-B and pro-B populations from Cbfb+/56M/Mx1Cre/H2-SVEX mice (restored) or control mice. The data represent results from at least 3 independent experiments. Numbers on plots are percentages of displayed cells.

Cbfβ-SMMHC hinders Rag1 and Rag2 expression, and V(D)J recombinase activity. (A) Relative expression levels of Rag1 and Rag2 in sorted CLPs by qRT-PCR. Shown are mean plus or minus SD (n = 3; *** P < .001) from at least 2 independent sorting experiments. (B) Illustration of the transgene H2-SVEX used in this study. The VEX reporter (white rectangle) in the antisense orientation flanked by V(D)J recombination signal sequences (triangles) that direct inversional recombination is driven by the murine H2K promoter (black rectangle). (C) Analysis of recombination reporter VEX expression in BM CLP populations from Cbfb+/56M/Mx1Cre/H2-SVEX mice (restored) or control mice. (D) Analysis of recombination reporter VEX expression in BM pre–pro-B and pro-B populations from Cbfb+/56M/Mx1Cre/H2-SVEX mice (restored) or control mice. The data represent results from at least 3 independent experiments. Numbers on plots are percentages of displayed cells.

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