Figure 2
The effects of blocking DC-derived activin-A with follistatin upon NK-cell IFN-γ production. Immature MoDCs were cultured at 5 × 104 cells in 96-well plates in media supplemented with IL-2 (20 U/mL) and GM-CSF (20 ng/mL) either alone or together with 105 autologous NK cells for 24 hours. (A) IFN-γ production in cocultures of MoDCs with various combinations (as shown) of IL-12 (10 ng/mL) and poly I:C (12.5 μg/mL) and/or NK cells and/or follistatin (400 ng/mL). (B) IFN-γ production by NK cells cultured with IL-2 and IL-12 with or without follistatin (400 ng/mL) for 24 hours. Data represent the mean plus or minus 1 SD of 3 separate donors. **P < .05 versus no follistatin.
