Figure 7
The effects of activin-A and TGF-β on NK-cell killing and IFN-γ production. Cytotoxicity of 2 × 104 calcein-labeled K562 or Jurkat cells by purified NK cells cultured at 106 in 24-well plates for 3 days in (A) media supplemented with IL-2 (100 U/mL) or (B) media supplemented with IL-2 (20 U/mL) + IL-12 (10 ng/mL) in the absence or presence of activin-A or TGF-β (each 100 ng/mL). Killing efficiency was determined by calcein dye release and measured by spectrometry. (C) NK-cell supernatants collected after the 3 days of culture were measured for IFN-γ production by ELISA. Data represent the mean plus or minus 1 SD of 3 separate donors. *P < .01 versus other groups.
