Effects of a function-blocking antiADAMTS13 monoclonal antibody (A10) on mural thrombus generation under very high shear rate conditions. Whole blood from healthy volunteers containing DiOC6 (1 μM)-labeled platelets, anticoagulated with argatroban, was perfused over a type I collagen-coated glass surface under very high shear rate (12 000 s−1) with anti-ADAMTS13 antibody A10 or with control mouse IgG (each 20 μg/mL). (A) Time-course changes of 3-dimensional images of thrombi (original magnification, ×600), which were constructed by the image-analyzing system of confocal laser scanning microscopy (CLSM) based on successive horizontal slices at identical portions, are representative of 10-pair flow experiments using blood from 10 independent donors. (B) Statistical analyses corresponding to the above images; bars represent mean (+ SD) surface coverage or total thrombus volume in 10 areas (each 133 × 100 μm) randomly selected in each perfusion using a single donor blood (donor number 1 in panel C). Note that thrombus generation is significantly (*; P < .01) accelerated in the presence of the anti-ADAMTS13 antibody. (C) Thrombus volume at 3 minutes' perfusion in 10-pair flow experiments using 10 independent donors; data points represent values of 10 areas randomly selected in each perfusion with (●) or without (○) anti-ADAMTS13 antibody, and transverse lines indicate mean values for each group. Note also that thrombus volumes generated in the presence of anti-ADAMTS13 antibody are significantly (P < .01; asterisks not included in the figure) greater than control thrombi in all 10-pair experiments.
