Dasatinib inhibits TCR-mediated activation of primary human T cells. (A) PBMCs were cultured with OKT3 in the absence (top) or presence (bottom) of dasatinib for 4 hours (original magnification, ×400). (B,C) PBMCs were stimulated with OKT3 (100 ng/mL) or PHA (0.5%) in the presence or absence of dasatinib (10 nM). T cells were analyzed for CD69 expression after 20 hours (B) or CD38 after 72 hours (C) by flow cytometry. Solid gray, OKT3 or PHA stimulation; dashed lines, stimulated T cells in the presence of 10 nM dasatinib; dotted lines, control.