LPS-induced IL-12 production is suppressed in PTEN^−/− BMDCs. (A) The overview of PI3K signaling pathway. PI(4,5)P₂ indicates phos-phatidylinositol(4,5)bisphosphate; PI(3,4,5)P₃, phosphatidylinositol-(3,4,5)tris-phosphate. (B) BMDCs from PTEN^−/− mice (n = 2) or their littermate controls (n = 2) were stimulated with 1 μg/mL LPS for 24 hours and assayed for the production of IL-12p70 and IL-10 by ELISA. Data are indicated as median plus or minus SD. Essentially, the same results were obtained with 2 independent experiments (experiments 1 and 2). The expression levels of PTEN and ERK2 in BMDCs were determined by Western blotting (right panel).