The effect of Rheb Q64L on LPS-induced cytokine production. BMDCs were infected with a lentivirus vector expressing a constitutively active form of Rheb (Rheb Q64L) or vector alone (control). Gene-transduced BMDCs were isolated (“Methods”) and stimulated with or without 1 μg/mL LPS for 24 hours. (A) The cell lysates were analyzed for Rheb, p70S6K, and ERK2 by Western blotting. Note that the mobility shifts of p70S6K caused by multiple phosphorylation are represented as a through d (Figure S4). (B) The production of IL-12p70 and IL-10 in culture supernatants was assayed by ELISA.