mTOR and GSK3β regulate LPS-induced IL-12 production through distinct mechanisms. (A) BMDCs were stimulated with 1 μg/mL LPS together with the indicated inhibitors for 24 hours and assayed for the production of IL-12p70 and IL-10 by ELISA. Data are indicated as median plus or minus SD of 3 independent experiments. (B) BMDCs from IL-10−/− mice were stimulated with 1 μg/mL LPS in the presence or absence of 5 μM SB216763 for 24 hours and assayed for the production of IL-12p70 by ELISA. Data are indicated as median plus or minus SD of 5 independent experiments. *P < .05 by Wilcoxon t test compared with LPS alone. (C) The schematic diagram of the PI3K-mediated regulation of IL-12 production. PA indicates phosphatidic acid; PI(4,5)P2, phosphatidylinositol(4,5)bisphosphate; PI(3,4,5)P3, phosphatidylinositol(3,4,5)trisphosphate.
