In vivo silencing of MINOR promoted DC survival. Mice received BM transplants with either LV-MINOR-siRNA– or LV-control-siRNA–transduced HSCs as described in “Bone marrow transplant and transduction of HSCs.” (A) Representative plots and an analysis of percentages of apoptotic DCs collected ex vivo during purification from spleens of those mice. (B) Representative plots and percentages of GFP-positive cells in total DC population in mice that received transplants showing that there was a bias toward retention of MINOR-inhibited CD11c⁺ cells. (C) Representative FACS plots of CD86 surface expression on DCs derived from the mice that received transplants indicating a higher level of CD86 expression in MINOR-inhibited CD11c⁺ cells. Statistical analyses were conducted on results of 2 separate experiments. *P < .05, analysis of variance or t test.