Induction of CCL3 and CCL4 protein expression in CLL-NLC cocultures. CLL PBMCs were placed in long-term cultures with outgrowth of NLCs, as described in “RNA isolation from CLL B cells before and after NLC coculture.” To determine the kinetics of induction of CCL3 and CCL4 protein expression, we analyzed supernatant samples at the time points indicated on the horizontal axis by ELISA. (A) The different symbols that are connected by the lines represent the mean CCL3 and CCL4 concentrations in 20 different CLL patients' samples at the indicated time points. In accordance with the microarray data, we found higher CCL4 protein expression compared with CCL3. (B,C) The individual protein expression data for CCL3 and CCL4 in the 20 CLL cases are displayed in dot plot diagrams. ZAP-70⁺ cases (•) can be distinguished from ZAP-70⁻ cases (○).