Figure 6
Effect of myr-RGT on thrombin-induced phosphorylation of integrin β3 cytoplasmic Y747 and Y759. Washed platelets were preincubated for 30 minutes with 250 μM of the different peptides as indicated. Thrombin (0.05 U/mL) was added to induce platelet aggregation with stirring at 1000 rpm for 1 minute. The platelets were then lysed in SDS-PAGE sample buffer and analyzed by Western blotting using monoclonal antibodies directed against the integrin β3 subunit extracellular domain (SZ21), β-actin, and polyclonal antibodies specific for the β3 integrin cytoplasmic sequences containing phosphorylated Y747 or Y759 residue, respectively.
