SHIP deficiency promotes the alteration of surface marker expression in the CD4⁺ T-cell compartment. Spleens and mesenteric LN of SHIP^ΔIP/ΔIP (n = 6) and WT (n = 6) littermates were harvested from 6- to 9-week-old mice and prepared into single-cell suspensions. Cells were then stained with and analyzed using fluorescently conjugated antibodies against CD3, CD4, CD25 with CD103, GITR, OX40, CD127, and FcγRII/III. (A) Representative histogram of CD103 expression levels on viable CD3⁺CD4⁺CD25⁺ (CD25⁺) or CD3⁺CD4⁺CD25⁻ (CD25⁻) T cells from spleen and LN of the indicated genotype. Bar graph representing percentage frequency of CD103⁺ T cells among viable CD25⁺ or CD25⁻ T cells from the spleen and LN of the indicated genotype. (B) Representative histogram of GITR expression on viable T cells as in panel A. Bar graph representing mean fluorescence intensity of GITR expression on viable CD25⁺ T cells from spleen and LN of the indicated genotype. Bar graph representing percentage frequency of GITR^hi (as determined by depicted gate in histogram) T cells among viable CD25⁻ T cells. (C) Same as in panel B, but for OX40 expression. (D) Representative histogram of CD127 (IL-7R) expression on viable T cells as in panels A to C. Bar graph representing mean fluorescence intensity of CD127 expression on viable CD25⁺ or CD25⁻ T cells from spleen and LN of the indicated genotype. (E) Same as in panel D, but for FcγRII/FcγRIII expression. *P < .05. **P < .01. ***P < .001.