Analysis of the signaling components in CD8 and CD4 T-cell subsets. Signaling components were quantified by RPP arrays and normalized to β-actin in the CD8 (A) and CD4 (B) T-cell subsets. The colors indicate the mean log₂ ratios of signaling components relative to N cells from 8 to 13 subjects. Signaling components showing significant differences (2-tailed paired t test; P < .05, shown in red) between CM and EM cells are represented as row ratios on individual graphs. CM and EM compartments are shown in black. Each set of linked dots corresponds to a distinct subject; n indicates the total number of subjects examined. Lck was detected using specific antibodies recognizing epitopes located at the N- and C-terminal ends of the protein (Lck N and Lck C, respectively) to ensure fully denaturing conditions.